Almad AA, Taga A, Joseph J, Welsh C, Patankar A, Gross SK, Richard JP, Pokharel A, Lillo M, Dastgheyb R, Eggan K, Haughey N, Contreras JE, Maragakis, N (2022). Connexin 43 hemichannels mediate spatial and temporal disease spread in ALS.  Proc. Natl. Acad. Sci. 119(13):e2107391119. doi: 10.1073/pnas.2107391119. 

Himelman E, Nouet J, Lillo MA, Chong A, Zhou D, Wehrens XHT, Rodney GG, Xie LH, Shirokova N, Contreras JE, Fraidenraich D (2022). A microtubule-connexin-43 regulatory link suppresses arrhythmias and cardiac fibrosis in Duchenne muscular dystrophy mice. Am. J. Physiol. Heat Cir. Physiol. Oct 7. doi: 10.1152/ajpheart.00179.2022.


Muñoz MF, Griffith TN, Contreras JE. Mechanisms of ATP release in pain: role of pannexin and connexin channels (2021). Purinergic Signal.  doi: 10.1007/s11302-021-09822-6. Epub ahead of print.

Jiang W, Lin YC, Botello-Smith W, Contreras JE, Harris AL, Maragliano L, Luo YL. Free energy and kinetics of cAMP permeation through connexin26 via applied voltage and milestoning (2021). Biophys J.  Aug 3;120(15):2969-2983. doi: 10.1016/j.bpj.2021.06.024.

Lillo MA, Contreras JE (2021). Opening the floodgates: An emerging role for Connexin-43 hemichannels in the heart. Cell Calcium Apr 30;97:102410. doi: 10.1016/j.ceca.2021.102410.

Seo JH, Dalal MS, Contreras JE. Pannexin-1 Channels as Mediators of Neuroinflammation (2021). Int J Mol Sci. May 14;22(10):5189. doi: 10.3390/ijms22105189.

Jiang W, Lin Y-C, Botello-Smith WM, Contreras JE, Harris A, Maragliano L, Luo Y (2021). Free energy and kinetics of cAMP permeation through connexin26 hemichannel with and without voltage. BioRxiv.

Narahari A, Kreutzberger AJB, Gaete PS, Chiu Y-H, Leonhardt SA, Medina CB, Jin X, Oleniacz PW, Kiessling V, Barrett PQ, Ravichandran KS, Yeager M, Contreras JE, Tamm LK, Bayliss DA (2021). Caspase-activated Pannexin 1 channels favor anionic molecules and support flux of ATP and other signaling metabolites. Elife. Jan 7;10:e64787. doi: 10.7554/eLife.64787.

Gaete PS,Contreras JE (2021). A method for assessing ionic and molecular permeation in connexin hemichannels. Methods in Enzymology. Ion Channels Part C Volume 653 (Editors, Minor and Colecraft). 654:271-293. doi: 10.1016/bs.mie.2021.01.009.


Almad AA, Taga A, Joseph J, Welsh C, Patankar A, Gross SK, Richard JP, Pokharel A, Lillo M, Dastgheyb R, Eggan K, Haughey N, Contreras JE, Maragakis, N (2020). Connexin 43 hemichannels mediate spatial and temporal disease spread in ALS. BioRxiv.

Gaete PS, Lillo MA, Lopez W, Liu Y, Harris AL, Contreras JE (2020). A novel voltage clamp/dye uptake assay reveals saturable transport of molecules through CALHM1 and connexin channels. J. Gen. Physiol. Nov2; 152(11):e202012607. 

Commentary at JGP: A new window into large-pore channels

Naulin PA, Lozano B, Fuentes C, Liu Y, Schmidt C, Contreras JE, Barrera NP (2020). Polydisperse molecular architecture of connexin 26/30 heteromeric hemichannels revealed by AFM imaging. J. Biol. Chem. Sep 4:jbc.RA119.012128. doi: 10.1074/jbc.RA119.012128. Epub ahead of print. PMID: 32887797.

Ho Seo J , Dalal MS , Calderon F, Contreras JE (2020). Myeloid Pannexin-1 mediates acute leukocyte infiltration and leads to worse outcomes after brain trauma. J. Neuroinflammation 17245.

Gaete PS, Contreras JE (2020). Ion Channels: Taking a close look at a large-pore channelElife Mar 31;9. pii: e56114. doi: 10.7554/eLife.56114.63.

Himelman E, Lillo MA, Nouet J, Gonzalez PJ, Zhao Q, Xie LH, Li H, Liu T, Wehrens XHT, Lampe PD, Fishman GI, Shirokova N, Contreras JE, Fraidenraich D (2020). Prevention of Connexin43 remodeling protects against Duchene muscular dystrophy cardiomyopathy. J. Clin. Invest. 128190. doi:10.1172/JCI128190.

Gaete PS, Contreras JE (2020). Uncoupled permeation through large-pore channels: ions and molecules don’t always ride together. J. Physiol. 598(2):209–210. doi:10.1113/JP279263.


Lillo MA, Himelman E, Shirokova N, Xie LH, Fraidenraich D, Contreras JE. S-nitrosylation of Connexin43 hemichannels elicits cardiac stress induced arrhythmias in Duchenne Muscular Dystrophy mice (2019). JCI Insight Dec 19;4(24). pii: 130091. doi: 10.1172/jci.insight.130091.

Valdez Capuccino JM, Chatterjee P, Garcia IE, Botello-Smith WM, Zhang H, Harris AL, Luo Y, Contreras JE (2019). The connexin26 human mutation N14K disrupts cytosolic inter-subunit interactions and promotes channel opening.   J. Gen. Physiol. 153(5):697-711.


Garcia IE, Villaleno F, Contreras GF, Pupo A, Pinto BI, Contreras JE, Pérez-Acle T, Alvarez O, Latorre R, Marinez AD, Gonzalez C (2018). The syndromic deafness mutation G12R impairs fast and slow gating in Cx26 hemichannels. J. Gen. Physiol.  150(5):697-711.

Garg C, Ho Seo J, Ramachandran J, Loh JM, Calderon F*, Contreras JE* (2018). Trovafloxacin attenuates neuroinflammation and improves outcome after traumatic brain injury in mice. J. Neuroinflammation.  15(1):42 (*Corresponding authors).

Gonzalez PJ, Ramachandran J, Badr MA, Kang C, Xie LH, Shirokova N*, Contreras JE*, Fraidenraich D* (2018). Normalization of connexin 43 protein levels rescues cellular and functional signs of dystrophic cardiomyopathy. Neuromuscular Disorders. 28(4):361-372  (*Corresponding authors).


Trease A, Valdez Capuccino JM, Contreras J, Harris A, Sorgen PL (2017). Intramolecular signaling in a cardiac connexin: Role of cytoplasmic dimerization. J. Molec. Cell. Cardiol. 111: 69-80.

Zamorano P, Marin N, Cordova F, Aguilar, Meininger C, Boric MP, Golenhofen N, Contreras JE, Sarmiento J, Duran WN, Sanchez FA (2017). S-nitrosylation of VASP at cysteine 64 mediates the inflammation-stimulated increase in microvascular permeability. Am. J. Physiol. Heart. Circ. Physiol. 313: H66-H71.

Ding Q, Heller B, Capuccino JM, Song B, Nimgaonkar I, Hrebikova G, Contreras JE, Ploss A (2017). Hepatitis E virus ORF is a functional ion channel requiered for release of infectious particles. Proc. Natl. Acad. Sci. 114: 1147-1152.


Lopez W, Ramachandran J, Alsamarah A, Luo Y, Harris AL, Contreras JE (2016). Mechanism of gating by calcium in connexin hemichannels. Proc. Natl. Acad. Sci. 113: E7986-E7995.

Capuccino JMV and Contreras JE (2016). Functional characterization of connexin hemichannels using Xenopus oocytes and the two-electrode voltage clamp technique. Methods in Signal Transduction. 203-214 (CRC Press).


Tong X, Lopez W, Ramachandran J, Ayad WA, Liu Y, Lopez-Rodriguez A, Harris AL, Contreras JE (2015). Glutathione release through connexin hemichannels: Implications on chemical modification for pores permeable to large molecules. J. Gen. Physiol. 146: 245-54.

Gonzalez PJ, Ramachandran J, Xie LH, Contreras JE, Fraidenraich D (2015). Selective Connexin43 inhibition prevents isoproterenol-induced arrhythmias and lethality in muscular dystrophy mice. Scientific Reports. doi: 10.1038/srep13490.

Garcia IE, Maripillan J, Jara O, Ceriani R, Palacios-Munoz A, Ramachandran J, Olivero P, Perez-Acle T, Gonzalez C, Saez JC, Contreras JE, Martinez AD (2015). Keratitis-Ichthyosis-Deafness syndrome-associated Cx26 mutants produce non-functional gap junctions but hyperactive hemichannels when co-expressed with wild type Cx43. J. Invest. Dermatol. 135: 1338-47. doi: 10.1038/jid.2015.20.


Harris AL and Contreras JE (2014). Motifs in the permeation pathway of connexin channels mediate Ca2+ and voltage sensing. Frontiers in Physiology. 31;vol 5:article 113. doi: 10.3389/fphys.2014.00113.

Lopez W, Liu Y, Harris AL, and Contreras JE (2014). Divalent regulation and intersubunit interactions of human Connexin26 (Cx26) hemichannels. Channels. 8(1):1-4. doi: 10.4161/chan.26789. Epub 2013 Oct 14.


Lopez W, Gonzalez J, Liu Y, Harris AL, Contreras JE (2013). Human pathogenic Connexin26 mutations (D50N/Y) reveals insights of the mechanisms of Ca2+ regulation of hemichannels. J. Gen. Physiol. 142:23-35. 


Miranda P*, Contreras JE*, Wesch D, Sigworth FJ, Holmgren M, Giraldez T (2012). State-Dependent FRET reports calcium- and voltage-dependent gating-ring motions in BK channels. Proc. Natl. Acad. Sci. 110: 5217-22 (*Equal contribution).


Contreras JE, Chen J, Lau AY, Roux B, Holmgren M (2010). Voltage profile along the permeation pathway of an open channel. Biophysical J. 99: 2863-69.

Contreras JE, Srikumar D, and Holmgren M (2008). Gating at the selectivity filter of cyclic nucleotide-gated channels. Proc. Natl. Acad. Sci. 105: 3310-14.

Contreras JE, and Holmgren M (2006). Access of quaternary ammonium blockers to the internal pore of cyclic nucleotide-gated channels: Implications for the location of the gate. J. Gen. Physiol. 127: 481-494. 

Contreras JE, Sánchez HA, Veliz L, Bukauskas FF, Bennett MVL, and Sáez JC (2004) Role of connexin-based gap junctions and hemichannels in ischemia-induced cell death in nervous tissue. Brain Res. Rev. 47: 290-303.

Contreras JE, Sáez JC, Bukauskas FF and Bennett MVL (2003) Gating and regulation of connexin 43 (Cx43) hemichannels. Proc. Natl. Acad. Sci. 100: 11388-93.

Contreras JE, Bukauskas FF, Sáez JC, and M.V. Bennett (2003). Functioning of Cx43 hemichannels demonstrated by single channel properties. Cell Communication and Adhesion.  10:245-9.

Bennett MVL, Contreras JE, Bukauskas FF, Sáez JC (2003) New role for astrocytes: Gap junction hemichannels have something to communicate. Trends in Neurosci. 26: 610-7.

Sáez JC, Contreras JE, Bukauskas FF, and M.V. Bennett (2003) Gap junction hemichannels in astrocytes of the CNS. Acta Physiol. Scand. 179: 9-22.

Contreras JE, Sánchez H, Eugenín EA, Speidel D, Theis M, Willecke K, Bukauskas FF, Bennett MVL and Saez JC (2002). Metabolic inhibition induces opening of unapposed connexin43 gap junction hemichannels and reduces gap junctional communication in cortical rat astrocytes in culture. Proc. Natl. Acad. Sci. 99: 495-500.

De Maio A, Vega VL, Contreras JE (2002). Gap junctions, homeostasis, and injury. J. Cell Physiol. 191: 269-282.

Brañes MC, Contreras JE, and Sáez J (2002). Human polymorphonuclear cells express connexins and form homologous gap junctions. Med. Sci. Monit. 8(8): BR313-323.

Sáez JC, Araya R, Brañes MC, Contreras JE, Eugenín EA, Martínez AD and Palisson F. Gap junctions in the native and memory immune system: Connexins regulation and possible functional roles (2000). Current Topics in Membranes. Ed. Peracchia C. Pp 555-579.